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Registro Completo |
Biblioteca(s): |
Embrapa Milho e Sorgo. |
Data corrente: |
01/12/2008 |
Data da última atualização: |
24/05/2018 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
MOTA, F. F. da; GOMES, E. A.; SELDIN, L. |
Afiliação: |
Fabio Faria da Mota, UFRJ; ELIANE APARECIDA GOMES, CNPMS; Lucy Seldin, UFRJ. |
Título: |
Auxin production and detection of the gene coding for the auxin effux carrier (AEC) protein in Paenibacillus polymyxa. |
Ano de publicação: |
2008 |
Fonte/Imprenta: |
Journal of Microbiology, v. 46, n. 3, p. 257-264, 2008. |
DOI: |
10.1007/s12275-007-0245-x |
Idioma: |
Inglês |
Conteúdo: |
Different species of Paenibacillus are considered to be plant growth-promoting rhizobacteria (pGPR) due to their ability to repress soil borne pathogens, fix atmospheric nitrogen, induce plant resistance to diseases and/for produce plant growth-regulating substances such as auxins. Although it is known that indole-3-acetic acid (lAA) is the primary naturally occurring auxin excreted by Paenibacülus species, its transport mechanisms (auxin effiux carriers) have not yet been characterized. In this study, the auxin production of P. polymyxa and P. graminis, which are prevalent in the rhizospheres of maize and sorghum sown in Brazil, was evaluated. In addition, the gene encoding the Auxin Effiux Carrier (AEC) protein from P. polymyxa DSM36T was sequenced and used to determine if various strains of P. polymyxa and P. graminis possessed this gene. Each of the 68 P. polymyxa strains evaluated in this study was able to produce IAA, which was produced at concentrations varyjng from 1 to 17 ug/ml. However, auxin production was not detected in any of the 13 P. graminis strains tested in this study. Dift'erent primers were designed for the PCR amplification of the gene coding for the AEC in P. polymyxa, and the predicted protein of 319 aa was homologous to AEC from Bacillus amyloliquefaciens, B. licheniformis, and B. subtilis. However, no product was observed when these primers were used to amplify the genomic DNA of seven strains of P. graminis, which suggests that this gene is not present in this species. Moreover, none of the P. graminis genomes tested were homol-ogous to the gene coding for AEC, whereas ali of the P. polymyxa genomes evaluated were. This is the first study to demonstrate that the AEC protein is present in P. polymyxa genome. MenosDifferent species of Paenibacillus are considered to be plant growth-promoting rhizobacteria (pGPR) due to their ability to repress soil borne pathogens, fix atmospheric nitrogen, induce plant resistance to diseases and/for produce plant growth-regulating substances such as auxins. Although it is known that indole-3-acetic acid (lAA) is the primary naturally occurring auxin excreted by Paenibacülus species, its transport mechanisms (auxin effiux carriers) have not yet been characterized. In this study, the auxin production of P. polymyxa and P. graminis, which are prevalent in the rhizospheres of maize and sorghum sown in Brazil, was evaluated. In addition, the gene encoding the Auxin Effiux Carrier (AEC) protein from P. polymyxa DSM36T was sequenced and used to determine if various strains of P. polymyxa and P. graminis possessed this gene. Each of the 68 P. polymyxa strains evaluated in this study was able to produce IAA, which was produced at concentrations varyjng from 1 to 17 ug/ml. However, auxin production was not detected in any of the 13 P. graminis strains tested in this study. Dift'erent primers were designed for the PCR amplification of the gene coding for the AEC in P. polymyxa, and the predicted protein of 319 aa was homologous to AEC from Bacillus amyloliquefaciens, B. licheniformis, and B. subtilis. However, no product was observed when these primers were used to amplify the genomic DNA of seven strains of P. graminis, which suggests that this gene is not pre... Mostrar Tudo |
Palavras-Chave: |
Auxin; Auxin efflux earrier protein; Indole-3-aeetic acid; Paenibacillus graminis. |
Thesaurus Nal: |
Paenibacillus polymyxa. |
Categoria do assunto: |
S Ciências Biológicas |
Marc: |
LEADER 02470naa a2200217 a 4500 001 1491541 005 2018-05-24 008 2008 bl uuuu u00u1 u #d 024 7 $a10.1007/s12275-007-0245-x$2DOI 100 1 $aMOTA, F. F. da 245 $aAuxin production and detection of the gene coding for the auxin effux carrier (AEC) protein in Paenibacillus polymyxa.$h[electronic resource] 260 $c2008 520 $aDifferent species of Paenibacillus are considered to be plant growth-promoting rhizobacteria (pGPR) due to their ability to repress soil borne pathogens, fix atmospheric nitrogen, induce plant resistance to diseases and/for produce plant growth-regulating substances such as auxins. Although it is known that indole-3-acetic acid (lAA) is the primary naturally occurring auxin excreted by Paenibacülus species, its transport mechanisms (auxin effiux carriers) have not yet been characterized. In this study, the auxin production of P. polymyxa and P. graminis, which are prevalent in the rhizospheres of maize and sorghum sown in Brazil, was evaluated. In addition, the gene encoding the Auxin Effiux Carrier (AEC) protein from P. polymyxa DSM36T was sequenced and used to determine if various strains of P. polymyxa and P. graminis possessed this gene. Each of the 68 P. polymyxa strains evaluated in this study was able to produce IAA, which was produced at concentrations varyjng from 1 to 17 ug/ml. However, auxin production was not detected in any of the 13 P. graminis strains tested in this study. Dift'erent primers were designed for the PCR amplification of the gene coding for the AEC in P. polymyxa, and the predicted protein of 319 aa was homologous to AEC from Bacillus amyloliquefaciens, B. licheniformis, and B. subtilis. However, no product was observed when these primers were used to amplify the genomic DNA of seven strains of P. graminis, which suggests that this gene is not present in this species. Moreover, none of the P. graminis genomes tested were homol-ogous to the gene coding for AEC, whereas ali of the P. polymyxa genomes evaluated were. This is the first study to demonstrate that the AEC protein is present in P. polymyxa genome. 650 $aPaenibacillus polymyxa 653 $aAuxin 653 $aAuxin efflux earrier protein 653 $aIndole-3-aeetic acid 653 $aPaenibacillus graminis 700 1 $aGOMES, E. A. 700 1 $aSELDIN, L. 773 $tJournal of Microbiology$gv. 46, n. 3, p. 257-264, 2008.
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Embrapa Milho e Sorgo (CNPMS) |
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Registro Completo
Biblioteca(s): |
Embrapa Arroz e Feijão; Embrapa Cerrados; Embrapa Unidades Centrais. |
Data corrente: |
10/07/2015 |
Data da última atualização: |
29/10/2021 |
Tipo da produção científica: |
Autoria/Organização/Edição de Livros |
Autoria: |
CORDEIRO, L. A. M.; VILELA, L.; KLUTHCOUSKI, J.; MARCHÃO, R. L. (ed.). |
Afiliação: |
LUIZ ADRIANO MAIA CORDEIRO, CPAC; LOURIVAL VILELA, CPAC; JOAO KLUTHCOUSKI, CNPAF; ROBELIO LEANDRO MARCHAO, CPAC. |
Título: |
Integração lavoura-pecuária-floresta: o produtor pergunta, a Embrapa responde. |
Ano de publicação: |
2015 |
Fonte/Imprenta: |
Brasília, DF: Embrapa, 2015. |
Páginas: |
393 p. |
Descrição Física: |
il. |
Série: |
(Coleção 500 perguntas, 500 respostas). |
ISBN: |
978-85-7035-453-2 |
Idioma: |
Português |
Conteúdo: |
Na forma de perguntas e respostas, um conjunto de informações relacionadas aos sistemas de integração lavoura pecuária floresta, que busca contribuir para o entendimento da importância desses sistemas, sua implantação, seus benefícios, suas particularidades regionais e principais desafios para ampliar sua adoção em todo o território brasileiro. Portanto, representa um rico acervo de informações práticas que se destinam aos empresários rurais e formadores de opinião que desejam conhecer e adotar adequadamente os sistemas de ILPF. |
Palavras-Chave: |
Agricultura conservacionista; Agrobusiness; Agroindustrial sector; Integração lavoura pecuária floresta; Integração lavoura-pecuária-floresta; Integração lavoura-pecuária-floresta (iLPF); Sistema agrossilvipastoril. |
Thesagro: |
Agronegócio; Agrossilvicultura; Desenvolvimento sustentável; Exploração agrícola; Exploração florestal; Integração; Lavoura; Pecuária; Sistema de produção. |
Thesaurus NAL: |
Environmental sustainability; Integrated agricultural systems; Sustainable development. |
Categoria do assunto: |
-- A Sistemas de Cultivo K Ciência Florestal e Produtos de Origem Vegetal P Recursos Naturais, Ciências Ambientais e da Terra |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/139643/1/cordeiro-01.pdf
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/215323/1/500-perguntas-integracao-lavoura-pecuaria.pdf
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/226884/1/90000033-ebook-epub-v2.epub
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Marc: |
LEADER 01868nam a2200409 a 4500 001 2022098 005 2021-10-29 008 2015 bl uuuu 00u1 u #d 020 $a978-85-7035-453-2 100 1 $aCORDEIRO, L. A. M. 245 $aIntegração lavoura-pecuária-floresta$bo produtor pergunta, a Embrapa responde.$h[electronic resource] 260 $aBrasília, DF: Embrapa$c2015 300 $a393 p.$cil. 490 $a(Coleção 500 perguntas, 500 respostas). 520 $aNa forma de perguntas e respostas, um conjunto de informações relacionadas aos sistemas de integração lavoura pecuária floresta, que busca contribuir para o entendimento da importância desses sistemas, sua implantação, seus benefícios, suas particularidades regionais e principais desafios para ampliar sua adoção em todo o território brasileiro. Portanto, representa um rico acervo de informações práticas que se destinam aos empresários rurais e formadores de opinião que desejam conhecer e adotar adequadamente os sistemas de ILPF. 650 $aEnvironmental sustainability 650 $aIntegrated agricultural systems 650 $aSustainable development 650 $aAgronegócio 650 $aAgrossilvicultura 650 $aDesenvolvimento sustentável 650 $aExploração agrícola 650 $aExploração florestal 650 $aIntegração 650 $aLavoura 650 $aPecuária 650 $aSistema de produção 653 $aAgricultura conservacionista 653 $aAgrobusiness 653 $aAgroindustrial sector 653 $aIntegração lavoura pecuária floresta 653 $aIntegração lavoura-pecuária-floresta 653 $aIntegração lavoura-pecuária-floresta (iLPF) 653 $aSistema agrossilvipastoril 700 1 $aVILELA, L. 700 1 $aKLUTHCOUSKI, J. 700 1 $aMARCHÃO, R. L.
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Embrapa Arroz e Feijão (CNPAF) |
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